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Higher-Throughput Protein Production and Characterization 2019

Thu, January 17 - Fri, January 18, 2019 San Diego, California
Hilton San Diego Bayfront 1 Park Blvd San Diego, California 92101 United States +1 619-564-3333

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About Higher-Throughput Protein Production and Characterization 2019

HIGHER-THROUGHPUT PROTEIN PRODUCTION AND CHARACTERIZATION, SAN DIEGO, JANUARY 17-18, 2019

High-throughput processing has come of age by transforming the traditional protein-by-protein trial-and-error approach for testing criteria and scaling up. In CHI’s Higher-Throughput Protein Production and Characterization conference, HTP is explored in the quest to develop methods that ensure quality and translate to large scale much more quickly and efficiently than in the past. Automation, robotics and liquid handlers will be discussed, along with developing small-scale models that shed light on bioproduction. Case studies will be presented that illustrate how leaders in the field are integrating HTP approaches to reduce the time and effort needed to successfully analyze proteins, fine tune processes, and achieve well-folded, pure protein.

Targeted experiments and molecular simulations will be used to shed light on the importance of protein surface clusters and ligand properties for creating selective separations in multimodal chromatography. Affinity precipitation using smart biopolymers for the simultaneous recovery and purification of both mAb and non-mAb biologics will then be presented. Finally, results will be given on a novel approach for the rapid development of integrated downstream biomanufacturing processes for biological products.

The success rate to identify a multi-specific lead molecule with favorable drug-like properties increases with the number of engineered variants tested. We recently established a novel, fully automated platform process for the in silico design and fast generation of large panels of multi-specific variants. Here, we report on the integration of miniaturized lab unit operations with cutting-edge automation for transient transfection, expression, purification and characterization of up to 10,000 engineered variants in high-throughput.

The presentation will focus on the setup of a modular expression toolbox, consisting of standardized elements influencing expression levels, which allow the rapid generation of multiple expression constructs and also the generation of complex expression optimization libraries. Advantages and implications of a modular cloning system including implementation into protein expression optimization workflows will be discussed and a number of successful case studies will be presented.

About Higher-Throughput Protein Production and Characterization 2018

HIGHER-THROUGHPUT PROTEIN PRODUCTION AND CHARACTERIZATION, SAN DIEGO, JANUARY 11-12, 2018

High-throughput processes have come of age by transforming the traditional protein-by-protein trial-and-error approach for testing criteria and scaling up. In this leading conference, HTP will be explored in the quest to develop methods that ensure quality and translate to large scale. Automation, robotics and liquid handlers will be discussed, along with developing small-scale models that shed light on bioproduction. Case studies will be presented that illustrate how leaders in the field are integrating HTP approaches to reduce the time and effort needed to successfully analyze proteins, fine tune processes, and achieve well-folded, pure protein.

This event will take place at Hilton San Diego Bayfront, 1 Park Blvd, San Diego, California.

Event Highlights:

  • Next-Gen HTP Tools and Technologies
  • HTP Purification
  • HTP ANTIBODY PRODUCTION
  • Assessing and Ensuring Developability
  • High-Throughput de novo Computational Protein Design and Its Applications
  • High-Throughput Protein Production within the Swedish Human Protein Atlas Project

 

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